This cluster was characterized by high expression of CCR6 or CELF2, genes that are commonly used in literature to define memory B cell precursors (Holmes et al., 2020). Although this cluster likely represents early MBCs derived from the LZ, we cannot exclude some technical issues or even MBC-GC doublet cells.
The annotation provided in this first version comprises 42 categories that provide a stable categories to classify single-cell transcriptomes of SLOs, useful for tools like Azimuth (see external URLs). In the next version, we will add a more detailed classification, encompassing all cell types and states identified in the tonsil atlas. A validation cohort was included to confirm the presence and accuracy of each annotation this latter level, using criteria such as cell neighborhood preservation, conservation of bona fide marker genes, and annotation confidence derived from the KNN classifier. For a full description and interpretation of this validation cohort, please refer to the final section of the manuscript.
