Single-nucleus transcriptomic profiling of human skeletal muscle from healthy adults

Regenerating/Immature Myonuclei

Ontology Term: skeletal muscle fiberCL:0008002
Synonyms Terms that have the same, or nearly the same, meaning as the selected cell type.: unknown
Category Class of which the cell type is a subclass.: myotubeCL:0002372
Ontology Assessment Disagreements with any aspect of the Cell Ontology.: —
Marker Gene Evidence Gene markers used by researcher to assign this cell annotation.: TTN, CMYA5, ABLIM2, MALAT1, NEAT1, MLIP
Canonical Marker Genes: MLIP, MYBPC1, NEB, SVIL, TRDN, TTN, ACTA1, DES, TNNC1
Negative Marker Genes Genes expected to be absent, or expressed at a notably low level, in these cells, and whose absence supports the assignment of this cell annotation.: —
Rationale: Regenerating/immature myonuclei are suggested by high levels of structural genes TTN, CMYA5, ABLIM2 together with nuclear RNAs MALAT1, NEAT1, PCNT, MLIP, and KIAA1217, with TTN and CMYA5 prominently upregulated relative to mature fibers.

Set of Labels Description: Cell annotations were generated with scType using marker genes curated from related publications, and then manually reviewed and adjusted based on the top highly variable genes in each Leiden cluster.
Annotation Method: both
Algorithm Name: scType
Algorithm Version: v1
Algorithm URL: https://github.com/IanevskiAleksandr/sc-type