The term fibroblast encompasses different stromal cell subpopulations. Single-cell technologies now enable granular definition of cell states, and shared fibroblast states across different human tissues in health and disease have been reported. However, a comprehensive analysis of fibroblast states across different disease contexts in a single organ has not been performed.
Here we utilise single-cell RNA sequencing from >300 000 human fibroblast cells in skin from health and 23 diseases to define fibroblast subpopulations. We identified 6 fibroblast subclusters in health and resolved their location in human skin tissue at single-cell resolution. In disease, we identified the presence of 3 novel states (F6: Myofibroblast, F6: Myofibroblast chemokine-high, and F7: Myofibroblast fascia-like) and polarisation of two cell states observed in healthy skinf rom disease (F1: Superficial regenerative and F3 CCL19+ Immune-interacting ADAMDEC1+/CXCL9+).
- Dataset Type
- Integrated. The file includes an integrated collection of datasets.
- Total Cells
- 337,376
- organism
- Homo sapiens
- Patient_status
- Healthy (129,321 cells)Psoriasis (47,323 cells)Dupuytren's (46,277 cells)SSc (27,444 cells)Keloid (24,113 cells)Lyme_disease/Erythema migrans (11,613 cells)Prurigo nodularis (11,573 cells)Eczema (8,739 cells)Non-keloid scar (6,435 cells)AA (5,429 cells)BCC (4,188 cells)SCLE (3,382 cells)Sarcoidosis (2,196 cells)Acne (2,051 cells)PSM (1,657 cells)Acral melanoma (1,451 cells)DLE (1,108 cells)HS (1,021 cells)SCC (812 cells)Drug reaction: DRESS (675 cells)Granuloma annulare (381 cells)Cutaneous melanoma (187 cells)
- disease
- normal (129,321 cells)psoriasis (47,323 cells)palmar fibromatosis (46,277 cells)systemic sclerosis (27,444 cells)keloid (24,113 cells)Lyme disease (11,613 cells)prurigo nodularis (11,573 cells)dermatitis, atopic (8,739 cells)cutaneous sclerosis (6,435 cells)alopecia areata (5,429 cells)basal cell carcinoma (4,188 cells)subacute cutaneous lupus erythematosus (3,382 cells)sarcoidosis (2,196 cells)acne (2,051 cells)localized scleroderma (1,657 cells)acral lentiginous melanoma (1,451 cells)discoid lupus erythematosus (1,108 cells)hidradenitis suppurativa (1,021 cells)squamous cell carcinoma (812 cells)drug rash with eosinophilia and systemic symptoms (675 cells)granuloma annulare (381 cells)cutaneous melanoma (187 cells)
- assay
- 10x 3' v2 (163,076 cells)10x 3' v3 (123,816 cells)10x 5' v2 (23,435 cells)10x 5' v1 (23,186 cells)10x 3' v1 (3,863 cells)
- tissue
- zone of skin
- Site_status_binary
- Lesional (172,469 cells)Nonlesional (164,907 cells)
- disease_category
- Nonlesional (164,907 cells)Scarring
(localised or generalised) (100,869 cells)Inflammatory without scarring (59,104 cells)Cancer (6,517 cells)Inflammatory with
risk of scarring (3,402 cells)Granulomatous (2,577 cells)
